Old School DNA Sequencing: Sanger method

In class we have been learning old school and new school methods of DNA sequencing. The old way was very tricky and on mistake can cause you to mess up all the genes. We had a normal control client and 3 patints. By doing this DNA sequencing activity you can determine if a disease is present by comparing them to the normal patient. I used the Codon Usage Table to figure out the codes for each patient to see if they all matched the normal patients. This is what we go off of to see if a person is normal and if not where it went wrong. On Abby, her DNA does not match the normal one; she has one gene and one protein that are different. This means she has a disease due to protein change, and she is refered to as a point mutation. Bob has one change in genes and ine in protein, which shows a diesease is present. He is refered to as point mutation and he is 3 amino acids short (shortened protein). Carol has seven gene changes and 6 protein changes, which shows she has a disease for sure. While looking at her DNA you can see that she has a frame shift mutation, which means she most likely has breast cancer. Down below are several pictures that show how I determined if there was a disease.